Shrimp are incapable of synthesizing the steroid ring. Many sterols and essential components such as molting hormones, sex hormones, bile acids and vitamin D, are synthesized from cholesterol. Cholesterol also functions as a component of membrane and in the absorption and transport of fatty acids. Therefore, cholesterol is considered as essential nutrient which must be provided in the diet (Akiyama et al., 1992).

Recommended levels of cholesterol in shrimp feeds range from 0.25% to 0.40%. Marine invertebrate meals and oils, i.e. squid, shrimp, clam, crab, are all excellent sources of cholesterol. The cholesterol content of squid meal and shrimp meal are 15% - 20% of the fat and 10% - 15% of the fat, respectively. Cholesterol supplements may be used when cost-effective (Akiyama et al., 1992).