| Cyst packaging conditions | |
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Cysts should be dehydrated before storage (moisture content preferably <5%) and kept away from humid conditions, since a higher moisture content reduces hatchability in time. Storage under vacuum or nitrogen is important, as the presence of oxygen will induce the formation of free radicals which eventually reduce hatchability. Properly packed cyst containers are preferentially kept at low temperature for acclimation. When frozen, the cysts should be held 1 week at room temperature before they are incubated in seawater for hatching (Dhont et al.,1993). | |