Ovarian maturation and spawning in laboratory environment
The second system, that of achieving ovarian maturation and fertile spawns in a laboratory environment, utilizes adult shrimp either captured in the wild or cultured, and exposes them to appropriate environmental conditions and nutrition to stimulate spawning. Unilateral eyestalk removal, or ablation, of females is used almost invariably as a requirement to obtain adequate levels of ovarian development. This system is demanding (and more so for some species than for others) in terms of water quality, disease prevention, nutrition, and environmental standards. Multiple spawnings are achieved from females, which are held for periods of three weeks to six months (Bray & Lawrence, 1992).

This system of larval production involves the achievement of ovarian maturation as well as spawning in a laboratory or greenhouse-type environment. This system is almost invariably dependent on use of unilateral eyestalk ablation of females (Bray & Lawrence, 1992).