Tamaru, C.S., C. Carlstrom-Trick, W.J. Fitzgerald, Jr., H. Ako-1996
Journal of the World Aquaculture Society, 27(4):363-
Abstract:
A high percentage (98.3%, N = 60) of the marbled grouper Epinephelus microdon individuals captured from spawning aggregations during July and August 1993 in the waters surrounding the island of Koror, Republic of Palau, Micronesia, were in the stage of maturity at which final maturation and spawning could be hormonally induced. The sex ratio of the captured fish was highly skewed towards males (4 male:1 female). Sexually immature females comprised the smallest size class, (<0.6 kg body weight (BW) or 33.0 cm total length (TL)), while sexually mature females were restricted to the 0.6-1.5 kg BW (33.0-46.4 cm TL) groups. Males predominated in size classes >0.6 kg BW, and individuals >1.5 kg BW (46.4 cm TL) were exclusively male. All females with oocytes that averaged (N = 50) >400 um in diameter were successfully induced to spawn by a two-injection protocol using human chorionic gonadotropin (HCG) at total dosages of 2,100-3,200 IU/kg fish. All males used in the spawning trials were administered a single injection of HCG at dosages of 500 or 1,000 IU/kg fish. Fecundity ranged between 7.96 x 10^5-1.24 x 10^6/kg BW, average spawned egg diameters ranged between 769-832 um, percent fertilization ranged between 32.6%-99.9%, and hatching percentages were >90.0%. Total fat content of eggs obtained from a pooled spawning event was 14.1 mg/100 mg dry weight. The data indicate that HCG is a suitable treatment for the induction of spawning in marbled grouper females that possess a mean oocyte diameter of 400 um or greater.
(Hawaii C's Aquaculture Consultant Services, 1157 Lunaapono Place, Kailua, Hawaii 96734 USA)
-----------------------------------------------------------------------------------