Kasornchandra, J., R. K.Y. Srikaew, J. S. J. Pounghaew, S.
Boonyaratpalin-1996
Abstract:
"Red disease with white patches" of Penaeus monodon is caused by Systematic Ectodermal and Mesodermal Baculovirus (SEMBV). Polymerase chain reaction (PCR) has been developed as a diagnostic tool for the detection of SEMBV infection in shrimp. A specific oligonucleotide primer was constructed from the sequence of the cloned SEMBV DNA fragment and tested for its specificity. Postlarva were screened for SEMBV infection using PCR at the National Institute of Coastal Aquaculture. Samples were treated with lysis buffer containing proteinase K. Nucleic acid preparation was carried out by phenol-chloroform extraction, followed by ethanol precipitation. Among 1,147 shrimp samples, only 53 samples or 4.62% gave positive amplification of the specific 520 bp product.
(National Institute of Coastal Aquaculture, Kaoseng, Songkhla 90000,
Thailand)
Editors Note - Systematic Ectodermal and Mesodermal Baculovirus (SEMBV) is now more commonly referred to as White Spot
Baculovirus (WSBV).