Gensler, A.L., Kennedy, S.B., Fernandez, E.M., Dutka-Gianelli, J., Tucker, J.W., Jr.
Abstract:
HBOI Bacillus no. 48 was administered to Artemia during two early stages in order to modify their microflora. Bacteria were isolated from treated and control Artemia and cultured on TSA-1% NaCl. Gram staining, 3% KOH test, and morphology were used for preliminary classification of the bacteria.
In the first experiment, Bacillus was added during incubation of Artemia cysts to investigate inhibition of bacterial contaminants (e.g., Staphylococcus, Micrococcus, and Vibrionaceae) commonly found on cysts. At hatching, treated nauplii had lower numbers of bacteria than controls.
The second experiment examined microfloral changes in metanauplii that had been fed either the test diet (algae, enrichment product, and Bacillus) or the control diet (algae and enrichment only). The test plates had fewer bacterial types than the controls, and a reduction in bacterial numbers was evident after 12-24 hours treatment with Bacillus.
(Harbor Branch Oceanographic Institution, 5600 North U.S. Highway 1, Fort Pierce, FL 34946, USA)
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