O.M. Millamena, M.N. Bautista, O.S. Reyes, A. Kanazawa-1997
Aquaculture, 151: 9-14
Abstract:
The threonine requirement was determined for juvenile marine
shrimp. Penaeus monodon postlarvae, PL20, were stocked in 30-1
fiberglass tanks at ten shrimp per tank arranged in a completely
randomized design with six replicates per treatment. They were fed
amino acid test diets (40% protein) with casein-gelatin as natural
protein sources and supplemented with crystalline L-amino acids to
simulate the amino acid profile of shrimp muscle except for
threonine. Graded levels of threonine were incorporated to obtain
0.72, 1.0, 1.28, 1.56, 1.84, and 2.12 g per 100 g diet or 1.8, 2.5, 3.2,
3.9, 4.6, and 5.3% of dietary protein. Relationship of weight gain with
dietary threonine level was analyzed by the quadratic regression
method to derive the threonine requirement. Results showed that the
quantitative threonine requirement for growth is 1.4% of the diet or
3.5% of dietary protein. This requirement for growth conforms with
the threonine level in the shrimp muscle.
(Aquaculture Department, Southeast Asian Fisheries Development
Center, Tigbauan, Iloilo 5021, Philippines)
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