EFFECTS OF DIETARY PROTEIN AND LIPID LEVEL ON WATER QUALITY, SURVIVAL AND GROWTH OF PENAEUS VANNAMEI POSTLARVAE.

M. Velasco, A. L. Lawrence, W. H. Neill

Abstract:

Static-water ecoassays were conducted to compare survival and

growth of Penaeus vannamei postlarvac (PL), and accumulation of

unassimilated nutrients in water. Feeds with four dietary protein

levels (10, 18, 25, and 33%) and three lipid levels (3,7, and 11%)

were used on a 4 x 3 factorial arrangement. PL of 1.2 mg average

weight were stocked at 1.5 PL/L (444 PL/m^2) and fed 15 times/day

during the 21-day experimental period. Feed was provided using the

following feeding curves:

y = 1.9509-0.1459x, +0.1085x^2 -0.0038x^3 (days 1 to 16) and

y = -8.0574+1.0094x (days 17 to 20), where y = mg of feed/PL and

x = day.

PL survival and biomass gain ranged from 76.6 to 100% (mean =

90%) and from 23.0 to 26.2%/day, respectively. PL survival was not

significantly different among diets. Growth was not significantly

different for feeds with 18% protein compared to feeds with higher

protein regardless of lipid level. Diets with 10% protein resulted in

significantly lower growth. Dissolved total inorganic nitrogen (TIN)

accumulation significantly increased with increasing protein level. At

the end of the growth trial mass-balance analysis accounted, on

average, for 87% of the nitrogen that had entered the culture system.

Assimilation of nitrogen into shrimp biomass averaged 85, 71, 48,

and 37% for feeds containing 10, 18, 25, and 33% protein,

respectively. Feed stability, and settleable solids accumulation in

culture tanks significantly decreased with increased lipid level.

These data indicate that dietary protein level may have a great impact

on nitrogen loading in the water. Moreover, high PL survival and

growth obtained with the 18% protein concurrent with minimum

dissolved TIN accumulation in the water warrants further research on

the development of environmentally friendly feeds.

(Shrimp Mariculture Research, Texas Agricultural Experiment

Station, Texas A&M University System, 1300 Port St., Port Aransas,

TX 78373 USA)

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