TAURA SYNDROME VIRUS AND REPRODUCTION OF PENAEUS VANNAMEI

Lotz, J.M., Ogle, J.T.

Abstract:

Taura Syndrome (TS) is the most important disease of farmed

penaeid shrimp in the Western Hemisphere and is responsible for

large production losses annually. The syndrome was first recognized

as a distinct disease in Ecuador in 1992 and subsequently it has been

identified in most shrimp farming regions of the Americas. TS is a

rapidly, progressing disease marked by extensive mortalities that

become evident 25-35 days after a shrimp pond is stocked with

shrimp. Elevated death rates last only a matter of days but commonly

reach 25% per day and leave a mere 5 - 25% of the shrimp alive.

Interestingly, the survivors of the mortality epidemics suffer no more

than normal mortality rates over the remainder of the production

cycle. The question remains as to whether the survivors of a TS

outbreak are genetically more resistant to TS virus than were those

animals that succumbed to an infection. If TSV survivors are

genetically more resistant then the offspring of a male and female

survivor would likely, be more resistant to a TSV challenge than

would animals that had not been exposed. To test this hypothesis

Penaeus vannamei that had survived a TSV infection and had been

grown to broodstock size in a commercial setting were acquired and

placed in a biosecure quarantine recirculating maturation facility. The

broodstock animals were held and subjected to screening for TSV

infections by bioassay. Although chronic infections are difficult to

detect most animals tested positive for TSV. Subsequently females

were ablated and reproduction ensued. A total of six mated females

were collected and placed in spawning tanks. All six spawned

fertilized eggs and the larvae were transferred to a quarantine larval

rearing. Four of the six spawns developed to postlarvae. The four

spawns were subjected to three bioassay screenings for TSV and

tested negative each time. In addition, the four spawns were

challenged with TSV and assessed for their susceptibility on two

separate occasions. The first TSV challenge found that three of the

four spawns were somewhat more resistant to TSV than a control

group. The second challenge produced similar results. The study

suggests that TSV is not transmitted vertically from female to

offspring through the oocytes and that offspring of TSV survivors

may be more resistant than offspring of animals not subjected to

infection.

(University of Southern Mississippi, Institute of Marine Sciences,

Gulf Coast Research Laboratory, P.O. Box 7000, Ocean Springs,

Mississippi, USA)

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