G. Merchie, P. Lavens, P. Sorgeloos-1997
Aquaculture, 155: 165-181, in Rotifer News, 30 (Dec '97)
PLC techniques were adapted and standardized for quantification of ascorbic acid (AA) and its derivatives in both diets and target organisms. In most of the species examined, the initial level of AA in Brachionus and Artemia impaired the determination of the AA requirements for optimal growth and survival. Results indicated that, within the concentration range tested, 20 mg AA/kg diet is sufficient for normal growth and survival. For production of postlarval shrimp, this level amounted to a minimum 20 and 130 mg AA/kg diet for P. monodon and P. vannamei, respectively, while a level of 2000 mg AA/kg diet was needed to enhance the resistance of shrimp postlarvae to stress conditions and bacterial infections.
(Univ. Gent, Lab. Aquaculture & ARC, Rozier 44, B-9000 Gent, Belgium)