D. Voltolina, M. Nieves, G. Navarro, T. Oliva, D. Peraza-1998
Aquaculture Engineering, 19(1): 7-15
Abstract:
Important discrepancies were obtained in growth experiments run with two diatoms and three agricultural fertilizers, using different protocols. With a short preacclimation to the new media and small inocula, the growth of Chaetoceros sp. was stunted and the cells were heavier than those of the f/2-grown control cultures. Without acclimation, growth was acceptable in the short term, but the cells were unable to grow if used as starter cultures for a new experiment with the same medium. With Phaeodactylum tricornutum, one of the fertilizers gave consistently equal or even better results than the control medium; with the other two media, growth was poorer and inconsistent. As with Chaetoceros, growth-limiting media resulted in cells which were heavier than normal. The results show the importance of the choice of the experimental protocol in growth experiments aiming to evaluate alternative, cheaper formulations of media for mass cultures. In these cases, the protocol should reproduce as close as possible the routines used for commercial mass production.
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