15 MAY 1999
EFFECT OF EXTENDERS ON SPERM CRYOPRESERVATION OF AFRICAN CATFISH, CLARIAS GARIEPINUS (BURCHELL)
B. Urbanyi, A. Horvath, Z. Varga, L. Horvath, I. Magyary, F. Radics-1999
Aquaculture Research, 30: 145-151
Abstract:
The effect of extenders was studied on the cryopreservation of sperm from African catfish, Clarias gariepinus (Burchell). The following six basic extenders were tested: fructose, glucose, sucrose, NaCl, KCl solutions and the artificial seminal plasma of the African catfish. Each of these extenders was tested both with and without buffer system (i.e. NaHCO3-CO2 and Tris-HCl) by using 10% dimethylsulphoxide (DMSO) as a cryoprotectant. The two-step freezing was carried out in a programmable freezer by using the following freezing rates: (1) 4 C /min between 3 and -4 C; (2) and 11 C/min between -4 and -80 C. The best post-thaw motility (25%) was achieved with 333 mmol/L fructose solution and NaHCO3 buffer. The fertilization experiments were carried out with unbuffered fructose and glucose extenders using various amounts of sperm and two fertilization methods: (1) dry and (2) wet. The best fertilization rates were achieved with 75 microL of sperm and wet fertilization with glucose extender, or 100 microL of sperm and dry fertilization in case of fructose - both methods fertilized 96% of all eggs.
(Laboratory of Biotechnology, Department of Applied Genetics and Breeding, Institute of Animal Husbandry, Godollo, University of Agricultural Sciences, H-2103 Godollo, Pater K. u. 1., Hungary, e-mail: urbanyib@nt.ktg.gau.hu)