ANTIBIOTIC RESISTANCE IN BACTERIA ISOLATED FROM ARTEMIA NAUPLII AND EFFICACY OF FORMALDEHYDE TO CONTROL BACTERIAL LOAD

A.S. Sahul Hameed & G. Balasubramanian-2000

Aquaculture, 183 (3-4): 195-205
Abstract:

A study was carried out to determine the antibiotic resistance in bacteria isolated from Artemia nauplii and the efficiency of formaldehyde to control the bacteria associated with Artemia nauplii. The total aerobic heterotrophic bacteria of Artemia nauplii was determined on seawater nutrient agar and TCBS agar, and ranged from 3.8×10^3 to 8.1×10^3 and 9.4×10^2 to 4.3×10^3 colony forming units (CFU) per nauplius on seawater nutrient agar and TCBS agar plates, respectively. Among these bacteria, 336 isolates were tested for their resistance to five antibiotics. The minimum inhibiting concentrations of chloramphenicol, erythromycin, nitrofurazone, oxytetracycline, tetracycline, formaldehyde and sodium hypochlorite for 336 isolates were recorded. The LC50 values of oxytetracycline, formaldehyde and sodium hypochlorite for Artemia nauplii were determined as 540.5, 293.1 and 5.6 mg/l, respectively, after 24 h of exposure. The efficacy of formaldehyde was compared with that of antibiotics and formaldehyde was found to be very effective in controlling the bacteria of Artemia nauplii.

(Aquaculture Division, Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509, Vellore Dist., Tamil Nadu, India)

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