DIVERSITY AND GENETIC DIFFERENTIATION IN ARTEMIA SPECIES AND POPULATIONS DETECTED BY AFLP MARKERS

Y. Sun, W.-Q. Song, Y.-C. Zhong, R.-S. Zhang, T. Abatzopoulos, R.-Y. Chen-1999

International Journal of Salt Lake Research, 8 (4): 341-350

Abstract:

Amplified Fragment Length Polymorphism (AFLP) markers were successfully employed to analyze 15 Artemia species and strains for genetic diversity. AFLP markers are extremely sensitive to even a small sequence variation. They are stable and more polymorphic than RAPD. Twelve pairs of primer combinations were used to detect AFLP bands, of which 384 were polymorphic, and DNA fingerprintings were obtained by using silver staining. The polymorphism analysis leads us to the following conclusions: 1. Artemia tibetiana seems to differentiate from A. sinica. 2. The parthenogenetic populations from inland salt lakes could follow an evolutionary path that is different from that of the coastal parthenogenetic populations.
(Department of Biology, Nankai University, Tianjin 300071, PR China)

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