CLONING AND EXPRESSION OF AN ELONGATION FACTOR-1 ALPHA IN SEA BREAM (SPARUS AURATA) LARVAE AND ADULT TISSUE

M.A. Nowell, D.M. Power, P.M. Guerreiro, L. Llewellyn, V. Ramsurn, T. Wigham, G.E. Sweeney-2000

Marine Biotechnology, 2 (2): 173-179 (from Current Contents)

Abstract:

A clone encoding the polypeptide elongation factor EF-1 alpha was isolated from a complementary DNA library prepared from sea bream (Sparus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1 alpha in other animal species, EF-1 alpha messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1 alpha appeared to have a relatively uniform distribution across all the tissues analyzed.

(Cardiff Univ, Cardiff Sch Biosci, POB 911, Cardiff CF1 3US, S Glam, Wales, UK, e-mail: SweeneyGE@cardiff.ac.uk)

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