Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers
P. Makridis, A.J. Fjellheim, J. Skjermo, O.
Vadstein-2000
Aquaculture International, 8 (5):367-380
Abstract:
Two bacterial strains, 4:44 and PB52, isolated from turbot (Scophthalmus
maximus L.) were used during a first feeding experiment with turbot larvae.
Bacteria were either added directly to the water on the day of hatching of
the larvae (day 0), or bioencapsulated in rotifers (Brachionus plicatilis)
distributed on day 2 after hatching. The two bacterial strains were found to
be present in the water of the rearing tanks throughout the experiment. The
addition of bacteria influenced the species-composition of the microflora
associated with intensively produced rotifers added to the tanks, and
resulted in colonization of the gut of the larvae by the added bacterial
strains. The strain 4:44 showed a peak on day 9 after hatching, and reached
a mean(sem) of 2.5(1.4) × 10^4 bacteria per larva, when added in a mixture
with PB52, and 0.4(0.1) × 10^4
bacteria per larva, when added alone. The strain PB52, on day 12 after
hatching, reached 5.2(1.5) × 10^4
bacteria per larva when added in a mixture with strain 4:44, and 12.5(0.7)
× 10^4
bacteria per larva, when added alone. The added bacteria colonized the gut
turbot larvae, whereas the bacterial land, the survival and growth of the
larvae were in most cases not influenced in a negative way by the addition
of bacteria.