Effects of polyunsaturated
fatty acids and gonadotropin on prostaglandin series E production in a
primary testis cell culture system for the European sea bass
J. F.
Asturiano, L. A. Sorbera, S. Zanuy, M. Carrillo-2000
Journal of
Fish Biology, 57(6): 1563-1574
Abstract:
Prostaglandin series E (PGE) production by
mixed populations of isolated sea bass Dicentrarchus labrax
testicular cells was measured after culture at 20°C in
L-15 for up to 24h in the absence or presence of arachidonic
acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic
acid (DHA) and/or gonadotropin (hCG). AA stimulated a
significant dose- and time-dependent increase in
PGE production. EPA stimulated PGE production only
during the first 6h although levels were 26.4% those
induced with AA; DHA reduced PGE production. Although
hCG alone had no effect, it enhanced AA-induced PGE
formation and suppressed EPA-induced PGE production.
These results suggest that essential PUFAs modulate
significantly testicular PGE production in vitro and
therefore may have important effects on steroidogenesis
and spermiation in the male European sea bass.
(Instituto de Acuicultura
de Torre de la Sal (CSIC), Ribera de Cabanes, 12595, Castellón, Spain)