A routine
method of DNA-extraction from extremely small metazoans, e.g. single rotifer
specimens for RAPD-PCR analyses
Ch. Leutbecher-2000
Hydrobiologia, 437 (1/3):133-137.
Abstract:
A simple and efficient method has been developed for isolating genomic DNA
from single specimens of extremely small metazoan animals, e.g. rotifers
(Rotifera: Monogononta). The amount and quality of the extracted DNA allows
the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to
be employed with up to three random primers per specimen. Reproducibility of
RAPD-fingerprints has been checked and verified. Exemplarily, RAPD patterns
of three Brachionus species (B. angularis, B. calyciflorus, B. urceolaris)
amplified with the primer OPA 07 are demonstrated.
(University
of Osnabrück, Spezielle Zoologie, D-49069 Osnabrück, Germany E-mail: Leutbecher@biologie.uni-osnabrueck.de)
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