AXENIC ARTEMIA CULTURE
From : Neil Auchterlonie naa@dml.ac.uk
To: Artemia Characterization artemia-l@email.sparklist.com
Date: 17 January 2001
QUESTION:
I need to produce bacteria-free cultures of Artemia
for some marine
fish larvae first feeding experiments.
Does anyone know how/if this has been achieved???
Dr. Neil Auchterlonie
Dunstaffnage Marine Laboratory,
P.O. Box 3,
Oban,
Argyll,
PA34 4AD, UK
Tel. +44 (0)1631 559000 (switchboard)
+44 (0)1631 559285 (direct line)
Fax +44 (0)1631 559001
e-mail: naa@dml.ac.uk
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COMMENTS 1:
You can find a useful description of a method
developed by D'Agostino in:
Author: Verschuere, L., Rombaut, G., Huys, G., Dhont, J., Sorgeloos, P., and
Verstraete, W.
Publication Date: 1999
Title: Microbial control of the culture of Artemia juveniles through
preemptive colonization by selected bacterial strains.
Journal: Applied and Environmental Microbiology
Volume: 65
Issue: 6
Pages: 2527-2533
This article can be retrieved as a pdf-file through our homepage but you'll
need to ask a password to my colleague Marc Verschraeghe. See:
http://allserv.UGent.be/~jdhont/pub/lijst.htm
and click on 'link'
Jean DHONT
Laboratory of Aquaculture & Artemia Reference Center
Ghent University - - Rozier, 44 - - 9000 Gent - - Belgium
tel: +32 9 264.37.54
fax: +32 9 264.41.93
E-mail : jean.dhont@UGent.be
site: http://www.UGent.be/aquaculture
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COMMENTS 2 :
I don’t know about bacteria-free but certainly
reduced levels can be achieved by utilizing A1 SELCO from INVE… Contact
them for further info.
Howard W. Newman
Desert Lake Technologies, LLC
E-mail: Bshrimp@aol.com
URL : www.desertlake.com
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COMMENTS 3:
Another method is using antibiotics, we have not
found any residues (by bioassays) of the antibiotics employed. We obtained
completely disinfected nauplii (by total viable count). The reference is:
Gomez-Gil, B., Ma.A. Herrera-Vega, A.F.
Abreu-Grobois, A. Roque-1998. Bioencapsulation of two strains of Vibrio
spp. in Artemia nauplii. Applied Environmental Microbiology, 64(6):
2318-2322.
Brune Gomez-Gil, Ph.D.
CIAD/Mazatlán Unit for Aquculture
AP. 711 Mazatlán Sin. 82000
Mexico
Tel: 52-69-880157
Fax: 52-69-880159
E-mail: bruno@victoria.ciad.mx