High-performance
liquid chromatographic determination of oxolinic acid and flumequine in the
live fish feed Artemia
M. Touraki, M.
Ladoukakis, C. Prokopiou-2001
Journal of Chromatography B, 751 (2) : 247-256
(from Current Contents)
Abstract :
A high-performance liquid chromatography (HPLC)
analytical method for the determination of oxolinic acid and flumequine in
Artemia nauplii is described. The samples were extracted and cleaned up by a
solid-phase extraction (SPE) procedure using SPE C-18 cartridges. Oxolinic
acid and flumequine were determined by reversed-phase HPLC using a mobile
phase of methanol-0.1 M phosphate buffer, pH 3 (45:55, v/v) and a UV
detection wavelength of 254 nm. Calibration curves were linear for oxolinic
acid in the range of 0.2-50 mug/g (r(2)=0.9998) and for flumequine in the
range of 0.3-50 mug/g (r(2)=0.9994). Mean recoveries amounted to 100.8% and
98.4% for oxolinic acid and flumequine, respectively. The quantification
limit was 0.2 mug/g for oxolinic acid and 0.3 mug/g for flumequine.
Quantitative data from an in vivo feeding study indicated excellent uptake
of both drugs by Artemia nauplii.
(Aristotelian Univ Salonika; Fac Sci; GR-54006
Salonika; Greece, e-mail : touraki@bio.auth.gr)