Effect of
various medium compositions on survival and hatching rates of embryos of the
giant freshwater prawn Macrobrachium rosenbergii cultured in vitro
P. Damrongphol, P. Jaroensastraraks, B.
Poolsanguan-2001
Fisheries Science, 67 (1) : 64-70
(from Current Contents)
Abstract :
The effectiveness of in vitro embryo culture of the
giant freshwater prawn Macrobrachium rosenbergii depended on the age of the
embryos at the onset of culture and on the concentrations of various
compositions in the medium. Embryos that started being cultured on day 0.5
after oviposition were more sensitive to variations in the medium
compositions than those that started being cultured on day 10.5 after
oviposition. An optimal NaCl level was essential for embryonic development,
survival, hatching and survival of the newly hatched larvae. Variations of
NaCl or KCI levels dramatically altered embryonic development, and variation
of the MgCl2 + MgSO4 level significantly lowered survival of the embryos
that started being cultured at the early stage of development. In contrast,
no significant change in embryonic development was observed upon variation
of the CaCl2 level. Hatching of the embryos required the presence of NaCl
and CaCl2 but not KCl or MgCl2 + MgSO4. The ionic requirements of the newly
hatched larvae differed from that of the developing embryos. Variations of
NaCl, KCI or CaCl2 but not MgCl2 + MgSO4 levels significantly influenced the
survival of the newly hatched larvae.
(Mahidol Univ, Fac Sci, Bangkok 10400, Thailand,
e-mail: scpdr@mahidol.ac.th)