Monitoring of the evolving diversity of the microbial community present in rotifer cultures
G.
Rombaut, G. Suantika, N. Boon, S. Maertens, P. Dhert, E. Top, P. Sorgeloos,
W. Verstraete-2001
Aquaculture, 198 (3-4): 237-252
Abstract:
The genetic fingerprint of the microbiota in the
culture water of two different rotifer culture systems (batch versus
recirculation) was obtained by means of denaturing gradient gel
electrophoresis (DGGE) of 16S rRNA gene fragments. The genetic profile of
the bacterial community present in the culture water of a batch culture
system changed daily due to shifts occurring at the level of the dominance
of bands. By means of statistical tools, it was possible to distinguish
three different periods during the development of the microbiota in the
recirculation experiment, each corresponding to a typical period during the
production of rotifers. Overall, it was obvious that the microbiota in such
a recirculation system was less susceptible for variation relative to the
microbiota in a batch system. However, some shifts in the genetic profile
were observed when technical problems occurred resulting in a reduced water
quality or performance of the biofilter.
Characterisation of the microbial community present
in the recirculation system indicated that the sequences of typical bands
showed the highest level of identity to the sequences from the bacterial
strain MMB-1T (representing a species in the genus Marinomonas,
Marinomonas mediterranea sp.), M. vaga and Pseudoalteromonas
haloplanktis spp. tetraodonis strain IAM 14160 (98%).
(Laboratory of Microbial Ecology and Technology (LabMET),
Ghent University, Coupure Links 653, 9000 Gent, Belgium, Tel.:
+32-9-264-5976; fax: +32-9-264-6248, e-mail of W. Verstraete: Willy.Verstraete@rug.ac.be,
URL: http://welcome.to/labmet)