Ovarian maturation and haemolymphatic vitellogenin concentration of Pacific white shrimp Litopenaeus vannamei (Boone) fed increasing levels of total dietary lipids and HUFA
Aquaculture Research, 32(7): 573-582
Abstract :
Two experiments were carried out to determine the
quantitative lipid requirements for ovarian maturation of Litopenaeus
vannamei (Boone) broodstock. The first experiment tested the effect of
total dietary lipid (TDL) levels of 8.1%, 8.8%, 9.8% and 11.2% on the
gonadosomatic index (GSI), hepatosomatic index (HSI), survival and body
weight. The second experiment tested the effect of total dietary levels of
highly unsaturated fatty acids (TDH) 0.6%, 1.3%, 2.0% and 2.7% on the same
parameters mentioned above and on the haemolymphatic vitellogenin (vg)
concentration. The GSI was higher in females fed 8.1% TDL as compared with
those fed 8.8% TDL (P </= 0.05), and higher in females
fed 8.1% and 8.8% TDL than in those fed 9.8% and 11.2% TDL. Increasing TDH
levels led to increasing levels of highly unsaturated fatty acids (HUFA) in
the ovaries (r2 = 0.999). However, the GSI was
not affected by dietary treatments (P > 0.05). The vg
concentration was higher in animals receiving 2.0% TDH as compared with
those receiving 2.7% TDH. In conclusion, high TDL levels affected ovarian
maturation in a negative way, why TDH levels had no pronounced effect on
ovarian maturation within the range tested in this study.
(Laboratory of Aquaculture & Artemia Reference
Center, Ghent University, Rozier 44, B-9000 Gent, Belgium)