immunity to viral
haemorrhagic septicaemia (VHS) following dna vaccination of rainbow trout at
an early life-stage
N.
Lorenzen, E. Lorenzen, K. Einer-Jensen-2001
Fish & Shellfish Immunology, 11(7): 585-591
Abstract:
Rainbow trout fry of average weight 0.5g
were vaccinated against viral haemorrhagic septicaemia (VHS)
by intramuscular injection of 1µg of plasmid DNA encoding
the VHS virus glycoprotein gene. Challenge with a
lethal dose of virus at two different time points, 9
and 71 days post-vaccination respectively, revealed
that a highly protective and lasting immunity was
established shortly after vaccination, in accordance
with earlier experiments with larger fish. The defence
mechanisms activated by the DNA vaccine are thus functional
at an early life-stage in rainbow trout.
(Danish Veterinary Laboratory, Hangøvej 2, DK-8200,
Aarhus N, Denmark, E-mail:nl@svs.dk)