Combined effect of temperature and food concentration on the grazing rate of the rotifer Brachionus plicatilis


D.J.S. Montagnes, S.A. Kimmance, G. Tsounis, J.C. Gumbs-2001

Marine Biology, 139(5): 975-979

Abstract:

We developed a predictive relationship to determine the grazing rate of Brachionus plicatilis at given temperatures and food concentrations; this function could be applied to experimental culturing and aquaculture practices. Grazing experiments were conducted at temperatures between 5°C and 40°C and at food concentrations, of the flagellate Isochrysis galbana, ranging between ~0 and 106 ml-1. In total, 136 grazing rates were determined, using the prey depletion method, for rotifers acclimated to treatments for 0.5 or 4 h. The response of grazing rate to temperature and food concentration was described using a model that combined a rectangular hyperbolic function for food concentration and a sigmoidal function for temperature. Using non-linear curve-fitting methods an equation was obtained: G=(45xF)/(159000+F)x 0.94/(1+219000xT-4.35), where G is the grazing rate (flagellates rotifer-1 min-1), F is the food concentration (flagellates ml-1), and T is temperature (°C). The equation indicates a maximum grazing rate of ~35 prey rotifer-1 min-1, above ~4x105 prey ml-1 and 25°C.

(Port Erin Marine Laboratory, University of Liverpool, School of Biological Sciences, Port Erin, Isle of Man IM9 6JA, UK, Tel: +44-1624-831008, Fax: +44-1624-831001, E-mail: dmontag@liv.ac.uk)

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