In
vitro culture of embryonic cells from the shrimp, Penaeus chinensis
Ting-Jun
Fan, Xiao-Feng Wang-2002
Journal
of Experimental Marine Biology and Ecology, 267(2): 175-184
Abstract:
Studies
were carried out in our laboratory to develop cell cultures from embryonic
tissue of Penaeus chinensis. Good yields of dissociated,
uncontaminated, viable cell suspensions were obtained by physical disruption
of harvested embryonic tissues. Primary cultures in the form of
proliferating foci of cells were readily initiated using MPS medium (osmolality:
2.4%) with 20% heat-inactivated fetal bovine serum. Insulin-like growth
factor-II (IGF-II) and basic fibroblast growth factor (bFGF) at different
concentrations were also added into the culture medium. Passage of primary
cultures resulted in rapid proliferation and good adherence of lymphocytes
in the presence of IGF-II at 80 ng/ml and bFGF at 20 ng/ml. Cells maintained
in subculture for up to 10 passages still had good cellular morphology and
division rates.
(Department
of Marine Biology, College of Marine Life Sciences, Ocean University of
Qingdao, 5 Yushan Road, Qingdao 266003, PR China, Tel.: +86-532-2032-459;
fax: +86-532-2032-276, e-mail: tjfan@ouqd.edu.cn)