Effect of live yeast
incorporation in compound diet on digestive enzyme activity in sea bass (Dicentrarchus
labrax) larvae
D. Tovar, J. Zambonino, C. Cahu, F.J. Gatesoupe, R. Lésel-2002
Aquaculture, 204(1-2): 113-123
Abstract:
Yeasts produce polyamines, and some strains have a
strong adhesion potential to intestinal mucus, an important condition for
probiotic efficiency. The aim of this study was to explore an in situ
production of polyamines by Debaryomyces hansenii HF1 (DH), a yeast
strain isolated from fish gut, in comparison with Saccharomyces
cerevisiae X2180 (SC) (Goteborg University Collection). The production
of polyamines by DH was three times higher than that of SC. The main
polyamines were spermine and spermidine, produced at a similar level. Both
strains adhered to the gut of sea bass larvae. When the yeasts were
introduced into a compound diet, the colonization was effective in the
larvae (104 CFU/g on a body weight basis). The DH diet led to an
increase in amylase secretion in 27-day-old larvae in comparison with the
control diet. The secretion of amylase and trypsin was lower in the SC diet,
and some delay in trypsin secretion was still observed in this group at day
42. At day 27, the activity of brush border membrane enzymes was stimulated
by the DH diet, and delayed by the SC diet, in comparison with the control
diet. The survival of the larvae was also increased in the DH diet, but the
growth rate was lower than that in the control group. This may be due to the
introduction of live yeast into the diet, which needs to be optimised.
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