Thiamin requirement of
juvenile abalone, Haliotis discus hannai Ino
W.
Zhu, K. Mai, G. Wu-2002
Aquaculture, 207(3-4): 331-343
Abstract:
Juvenile abalone, Haliotis discus hannai Ino,
of initial weight 0.55±0.02 g, were fed with experimental diets with graded
levels of thiamin-HCl (0, 9.8, 18.9, 37.2, 75.9, 150.4 and 309.5 mg/kg diet)
for 16 weeks. Thiamin-HCl and other water-soluble vitamins were
microencapsulated with sodium alginate by an emulsion coacervation process
prior to supplementation. To estimate the thiamin requirement of abalone and
to evaluate the thiamin status in this animal, weight gain (WG), daily
increment in shell length (DISL), tissue thiamin, thiamin pyrophosphate
(TPP), thiamin monophosphate (TMP), transketolase (TKA) and thiamin
pyrophosphate effect (TPP-effect) both in viscera and muscle were measured.
Except for TMP, all parameters mentioned above responded significantly to
the change of dietary thiamin levels in a broken line model. However, growth
(WG and DISL), TPP concentrations and TPP-effect both in viscera and muscle
were found to be the most sensitive indicators. Based on broken line
analyses, the thiamin requirement to satisfy maximum growth was estimated to
be 51 mg/kg diet, and the dietary thiamin levels to maximize visceral TPP
content and TPP-effect of H. discus hannai were estimated to be 61
and 58 mg/kg diet, respectively.
(Mariculture Research Laboratory, College of
Fisheries, Ocean University of Qingdao, Qingdao 266003, People's Republic of
China, Tel.: +86-532-2032282; fax: +86-532-2032799, e-mail of K. Mai:
kmai@mail.ouqd.edu.cn)