Bath immunisation of spawn, fry and fingerlings of Indian major carps using a particulate bacterial antigen

P. Swain, S.K. Nayak, A. Sahu, B.C. Mohapatra, P.K. Meher-2002    
Fish & Shellfish Immunology, 13(2): 133-140

Abstract:
Larval mortality in Indian major carps is one of the major problems encountered in the pond culture system. The present investigation was carried out to investigate the proper age, duration of exposure, and optimum bacterin concentration for vaccinating rohu (Labeo rohita) and catla (Catla catla) at their early stages with a formalin killed Edwardsiella tarda bacterin suspension. The development of immunological competence was recorded with spawn of rohu and catla of 3 weeks of age exposed to a bacterin at a concentration 10⁹cfu ml^-1 for 15min, where it persisted up to 4 weeks post vaccination. They showed significant resistance against challenge with virulent E. tarda bacteria. Significant antibody titre could be recorded in advanced fries and fingerlings exposed to 10⁹cfu/ml^-1 bacterin concentration for 45 and 60min, respectively.

(Aquatic Animal Health Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, India)

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