Inhibitory effect of sodium bicarbonate on the motility of sperm of Japanese eel


S. Tanaka, H. Zhang, Y. Yamada, A. Okamura, N. Horie, T. Utoh, N. Mikawa, H.P. Oka, H. Kurokura-2002

Journal of Fish Biology, 60(5): 1134-1141

Abstract:

Sperm motility of Japanese eel Anguilla japonica was depressed from 59·1 to 1·1% when NaHCO3 concentration increased from 0 to 150m M. In 25m M NaHCO3, when pH of the medium was 6·2, 7·2, 8·2, 9·2 and 10·2, relative sperm motility was 0, 0, 55·8, 93·7 and 136·6%, respectively to that of the control (0m M NaHCO3). The remarkable effect in acid or neutral condition indicates that free-CO2 (liberated CO2 and H2CO3) is a key factor for the motility inhibiting effect of NaHCO3.

(IRAGO Institute, Shinden 377-Ehima, Atsumi-cho, Atsumi-gun, Aichi, 441-3605, Japan, Tel.: +81 531 37 1680; fax: +81 531 34 3111; email: tanaka@irago.co.jp)


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