Phototrophic bacteria as
feed supplement for rearing Penaeus monodon larvae
S. Al-Azad, V.C. Chong, S. Vikineswary-2002 AU
Journal of the World Aquaculture Society, 33(2):
158-168 (from Current Contents)
Abstract :
Larvae of the giant tiger prawn Penaeus monodon were
reared from the naupliar to postlarval stage, based on a diet of waste-grown
phototrophic bacterium Rhodovulum sulfidophilum, the marine diatom
Skeletonema costatum, and brine shrimp Artemia. The experimental design
consisted of feeding the protozoea with diatoms and varying proportions of
bacterial biomass as diet D0 (0%), D1 (1%), D2 (2%), D3 (3%), and D5 (5%),
while mysis and postlarvae were fed the above diet supplemented with Artemia
nauplii. Controls were not fed with bacteria biomass (diet DO). The addition
of R. sulfidophilum biomass in the diet had significant effects on the
growth and survival of P. monodon larvae. In the first experiment, the
larvae fed with diet D1 (Skeletonema + 1% R. sulfidophilum) reached the
first postlarval stage (PL1) on the ninth day of feeding and had a mean
total length (TL) of 6.13 +/- 0.05 mm, which was significantly higher (P
< 0.001) than TL of larvae fed with the other three diets. In another
experiment, prawn larvae fed with diet D2 had the highest overall survival
of 46% and a significantly larger mean TL of 6.88 +/- 0.18 mm at PL1
compared to larvae fed with diet D0 and D3 (P > 0.05). However, in both
experiments prawn larvae fed with the various diets showed significant
differences in their length-at-stage (P > 0.05), and the lowest growth
and survival were obtained from diets comprising 3% and 5% bacterial
biomass, which also gave the highest levels of ammonia-nitrogen in the
culture water. Addition of more than 2% R. sulfidophilum biomass apparently
deteriorates the quality of the culture water.
(Univ Malaya, Inst Biol Sci, Kuala Lumpur 50603, Malaysia)