Influence of different
dietary amino acid patterns on growth and body composition of juvenile
Japanese flounder, Paralichthys olivaceus
S. Alam, S.-i. Teshima, D. Yaniharto, S. Koshio, M.
Ishikawa-2002
Aquaculture, 210(1-4): 359-369
Abstract:
A feeding trial using five semi-purified diets (50%
crude protein) was conducted to investigate the effects of different dietary
amino acid patterns on growth and body composition of juvenile Japanese
flounder. The control diet contained casein and gelatin as intact protein
sources and four other diets contained 30% casein-gelatin (2:1, w/w) and 20%
crystalline amino acids (CAA). CAA were added to the diets to simulate the
amino acid pattern found in red sea bream egg protein (REP), Japanese
flounder larvae whole body protein (FLP), Japanese flounder juvenile whole
body protein (FJP), and brown fish meal protein (BFP), respectively. The
test diets were fed to triplicate groups of juveniles (2.75±0.05 g) twice a
day for 40 days to evaluate weight gain, survival, feed conversion
efficiency (FCE), protein efficiency ratio (PER), and apparent protein
utilization (APU). The apparent retention of total dietary amino acids in
the whole body and A/E ratios of the whole body were also evaluated. The
highest weight gain was observed in fish fed the diet containing the dietary
amino acid pattern of BFP followed by fish fed the control, FJP, FLP and the
REP diets. Percent survival, FCE, PER and APU were also significantly (P<0.05)
affected by the amino acid pattern in the diets, indicating the highest
value in fish fed the BFP diet. Except for a few amino acids, the amino acid
composition of the whole body did not show marked differences with different
dietary amino acid pattern. Results suggest that BFP could be more suitable
as a reference amino acid pattern in the diet of juvenile Japanese flounder
compared to the amino acid pattern of FLP, FJP or REP.
(Laboratory of Aquatic Animal Nutrition, Faculty of
Fisheries, Kagoshima University, Shimoarata 4-50-20, Kagoshima 890-0056,
Japan, Tel.: +81-99-286-4181; fax: +81-99-286-4184, e-mail of: S.-i.
Teshima: teshima@fish.kagoshima-u.ac.jp)