Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

J. Glogowski, R. Kolman, M. Szczepkowski, Á. Horváth, B. Urbányi, P. Sieczyński, A. Rzemieniecki, J. Domagała, W. Demianowicz, R. Kowalski, A. Ciereszko-2002 

Aquaculture, 211(1-4): 367-373
Abstract:

Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris-sucrose-KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris-NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris-sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris-sucrose-KCl, Tris-NaCl, and Tris-sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris-sucrose-KCl and Tris-NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.

(Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland, Tel.: +48-89-535-7426; fax: +48-89-535-7421, e-amil of A. Ciereszko: acieresz@pan.olsztyn.pl)

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