The effect of diet on the biochemical composition of
juvenile Artemia: Potential formulations for rock lobster aquaculture
M.M.
Nelson, B.D. Mooney, P.D. Nichols, C.F. Phleger, G.G. Smith, P. Hart, A.J.
Ritar-2002
Journal
of the World Aquaculture Society, 33(2): 146-157 (from Current Contents)
Abstract :
The
lipid class and fatty acid (FA) composition of juvenile Artemia fed
continuously on four diets-the microalga Tetraselmis suecica, a mix of oat
bran-wheat germ-lecithin (OWL), OWL-eicosapentaenoic acid (EPA), and
OWL-EPA-arachidonic acid (AA)-were examined over a 9-d experiment in an
attempt to approximate the FA profile of phyllosoma larvae of wild southern
rock lobster Jasus edwardsii. The main difference in lipid class composition
of Artemia fed the four diets was the relative level of polar lipid (PL) and
triacylglycerol (TAG). By day 9, the algal-fed Artemia were highest in PL
(95% of total lipid) and lowest in TAG (2%), whereas the remaining diets
resulted in Artemia with 16-30% PL and 41-82% TAG. After 2 d, the relative
FA composition of all Artemia treatments closely reflected those of the
diets, with no marked change after further feeding (to day 9). In terms of
the content of essential polyunsaturated fatty acids (PUFA), by day 5
Artemia fed: 1) with the algal diet contained 7 mg/g FA dry mass (0.3% DHA,
6.3% EPA, 3.4% AA of total FA); 2) with the OWL diet contained 3 mg/g (0.3%
DHA, 0.9% EPA, 0.7% AA); 3) with the OWL-EPA diet contained 55 mg/g (6.2%,
DHA, 11.6% EPA, 1.1% AA); and 4) with the OWL-EPA-AA contained 83 mg/g (3.8%
DHA, 7.5% EPA, 17.4% AA). The PUFA profiles of Artemia using the OWL-oil
diets were similar to wild rock lobster phyllosomata, although levels of
docosahexaenoic acid (DHA) were lower (10% DHA) than in J. edwardsii larvae.
On the basis of PUFA composition data alone, the results suggest the
suitability of the OWL-oil mixed diets for consideration for feeding to
Artemia used in the culture of southern rock lobster larvae, particularly if
the level of DHA can be further enhanced.
(Univ
Tasmania; Dept Zool; GPO Box 252-05; Hobart; Tas 7001; Australia)