In vitro approach to studying the mechanisms of oocyte maturation in
sturgeons: a review of fundamental and applied aspects
B. F. Goncharov-2002
Journal of
Applied Ichthyology, 18(4-6): 368-374
Abstract:
This review summarizes the results of our
many-years use of the model of in vitro sturgeon oocyte maturation
for different purposes, such as comparison of gonadotropic activities of
different preparations, development of a criterion for the selection of
females for breeding, and monitoring of the developmental stage and/or
physiological state of ovarian follicles for studying the effects of
different factors in order to improve the breeding technology. Special
attention is paid to the conditions of in vitro ovarian follicle
manipulation since they can affect the results of experiments and their
interpretation. We also discuss two phenomena: the inhibitory effect of
gonadotropin on the progesterone-induced oocyte maturation and the
non-hormonal induction of oocyte maturation, further studies of which can
elucidate the mechanisms underlying the hormonal regulation of oogenesis in
sturgeons.
(Kol'tsov Institute of Developmental
Biology, Russian Academy of Sciences, 26 Vavilov St., Moscow, 119991 Russia.
E-mail: bfgon@mail.ru)