Sex steroid profiles in female and male stellate sturgeon (Acipenser stellatus Pallas) during final maturation induced by hormonal treatment


T. Semenkova, I. Barannikova, D.E. Kime, B.G. McAllister, L. Bayunova, V. Dyubin, N. Kolmakov-2002

Journal of Applied Ichthyology, 18(4-6): 375-381

Abstract: 

Serum cortisol, testosterone, 11-ketotestosterone (11KT), oestradiol-17β, progesterone, 17,20 β, 21-trihydroxy-4-pregnen-3-one (20 β S) concentrations were measured by enzyme-linked immunosorbent assay in stellate sturgeon (Acipenser stellatus Pallas) held at the fish farm during final maturation induced using pituitary preparation (PP) in females and luteinizing hormone-releasing hormone analogue (LH-RH-A) in males. Testosterone had surged by 5 h after the first injection of PP (5% of total dose), tended to increase after the second injection of PP (95% of total dose) and then the sharp decrease in females was followed by ovulation. Serum 20 β S levels in females increased dramatically after the second injection of PP and decreased sharply after ovulation. Following hormonal treatment, 11KT levels decreased slightly in females, but increased significantly in males 24 h after LH-RH-A injection. Mean 11KT concentrations were significantly higher in males than in females. The results suggest that in sturgeon 11-KT is the dominant androgen and 20 β S the maturation inducing steroid.

(Central Laboratory of Fish Reproduction, 24 Prof. Papova, St Petersburg 197022, Russia E-mail: cpl@it4257.spb.edu)


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