Analysis of
genetic variation in the Chinese sturgeon, Acipenser sinensis:
estimating the contribution of artificially produced larvae in a wild
population
B. Zhu, F. Zhou, H. Cao, Z. Shao, N. Zhao,
B. May, J. Chang-2002
Journal of
Applied Ichthyology, 18(4-6): 301-306
Abstract:
Twenty-five sets of microsatellite primers
developed from lake sturgeon (Acipenser fulvescens) and shovelnose
sturgeon (Scaphirhynchus platorynchus) genomic DNA were tested on
Chinese sturgeon, Acipenser sinensis. Ten sets of primers
successfully produced resolvable amplicons, and four of these sets (Afu-39,
Afu-54, Afu-68, and Spl-168) were used to analyze genetic variation in
mature adults, juveniles, and a single family of Chinese sturgeon from the
Yangtze River. Offspring from the single family were stocked prior to the
juvenile sample being taken. Results from genotyping parents and offspring
indicated that all four loci appear to be tetrasomic and all alleles appear
to segregate among the offspring in a 1 : 1 (presence : absence)
ratio. Neighbor-joining based on band-sharing in coupling with parentage
analysis revealed that hatchery propagated individuals may comprise 5-10% of
the juvenile population from a stocking of 30 000-60 000 larvae.
(Jianbo Chang, Institute of Hydrobiology,
Chinese Academy of Sciences, Wuhan 430072, China. E-mail of J. Chang: jbchang@ihb.ac.cn)