Virulence of luminous
vibrios to Artemia franciscana nauplii
S.A.
Soto-Rodriguez, A. Roque, M.L. Lizarraga-Partida, A.L. Guerra-Flores,
B. Gomez-Gil-2003
Diseases of Aquatic Organisms, 53: 231-240
Abstract:
From healthy and diseased penaeid shrimp from Asia
and the Americas, 25 luminous and 2 non-luminous bacterial strains were
isolated, and 14 were phenotypically identified as Vibrio harveyi;
9 isolates produced significant mortalities (45 to 80%) in Artemia
franciscana nauplii at inoculation densities of 105 to 106
CFU ml-1 compared to the controls (unchallenged nauplii). The
maximum number of bacteria ingested (bioencapsulated) by the Artemia
nauplii varied from less than 10 to 103 CFU nauplius-1
and no significant relationship was observed between the density of bacteria
inoculated, the amount of bacteria ingested, and naupliar mortality.
Significant correlations were obtained between naupliar mortality and
production of proteases, phospholipases or siderophores, but not between
mortality and lipase production, gelatinase production, hydrophobicity or
hemolytic activity. The results suggest that virulence of the strains tested
was more related to the production of particular exoenzymes than to the
measured colonization factors.
(Department of Marine Biotechnology, Centro de
Investigación Científica y de Educación Superior de Ensenada (CICESE),
Ensenada 22860, Baja California, Mexico, e-mail of B. Gomez-Gil: bruno@victoria.ciad.mx)