Control
of Vibrio alginolyticus in Artemia culture by treatment with
bacterial probiotics
L.
Villamil, A. Figueras, M. Planas, B. Novoa-2003
Aquaculture, 219(4): 43-56
Abstract:
In
order to determine the influence of six lactic acid bacterial (LAB) strains,
lactic acid and major extracellular products (ECPs) of LAB on the bacterial
load of Artemia culture, two experiments were carried out. In both
experiments, the dominant bacterial species were identified as Vibrio
alginolyticus. The treatment with Lactobacillus brevis (1×108
bacteria/ml) and lactic acid (0.1 g/l) reduced the load of this bacterium in
the Artemia culture water.
Biochemical
and morphological characteristics of the V. alginolyticus strains
isolated from Artemia culture were determined. For comparative
purposes, another V. alginolyticus strain isolated from diseased
turbot larvae was also included.
The
in vitro antibacterial activity of extracellular products (ECPs) from LAB,
as well as lactic acid (0.1 and 0.05 g/l), was assessed against both V.
alginolyticus strains. ECPs from LAB were able to inhibit the growth of V.
alginolyticus, especially those from L. brevis. In the case of
lactic acid treatments, no significant inhibition of V. alginolyticus
growth was observed.
(Instituto
de Investigaciones Marinas, Consejo Superior de Investigaciones Cientificas
(CSIC), Eduardo Cabello, 6 Marinas, 36208, Vigo, Spain, e-mail of B. Novoa: virus@iim.csic.es)