Disinfection of Sparus
aurata eggs with glutaraldehyde
A.M. Escaffre, D. Bazin, P. Bergot-2001
Aquaculture
International, 9(5):
451-458
Abstract:
This study was conducted to determine suitable
conditions for disinfecting eggs of gilthead sea bream (Sparus aurata)
with glutaraldehyde. Effects of the developmental stage (4–8
cells, morula, blastopore closure or heart beating), of the concentration (C
= 200, 300 or 400 ppm) and of the duration of the glutaraldehyde treatment
(T = 2 to 10 min) were investigated. Before the blastopore closure stage,
egg manipulation and treatment induced mortality. After this stage, the
toxicity of the glutaraldehyde treatment was negligible if the value of the
product C × T was less than 1000. Above this
value, the percentage of hatching and of normal larvae decreased and the
percentage of imprisoned larvae increased. Toxic effects of glutaraldehyde
varied according to the egg quality at the time of the treatment. It was
concluded that 200 ppm glutaraldehyde for 4 min, at the blastopore closure
stage or at the heart beating stage, were acceptable conditions for
disinfecting gilthead sea bream eggs at 18 °C.
(Station d'Hydrobiologie INRA,
Unité mixte INRA-IFREMER de Nutrition des Poissons, B.P.3,
Saint-Pée-sur-Nivelle, France, e-mail : escaffre@st-pee.infra.fr)