Detection of monodon baculovirus and white spot syndrome virus in apparently healthy Penaeus monodon postlarvae from India by polymerase chain reaction


S.K. Otta, I. Karunasagar, I. Karunasagar-2003
Aquaculture, 220(1-4): 59-67
Abstract:

The simultaneous presence of monodon baculovirus (MBV) and white spot syndrome virus (WSSV) in apparently healthy postlarvae of Penaeus monodon from different hatcheries in India was studied by nested polymerase chain reaction (PCR). MBV could be detected in 54% of the samples. However, only 15% of samples were positive by non-nested reaction. WSSV could be detected in 75% of samples, 19% being positive by non-nested reaction. The results show simultaneous presence of WSSV and MBV in many samples at various degrees of infection. Only 14% of the samples analysed were negative for both viruses.

(Department of Fishery Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore 575 002, India, e-mail of I. Karunasagar: mircen@sancharnet.in or karuna8sagar@yahoo.com) 

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