vibrio
neptunius sp. nov., vibrio brasiliensis sp. nov. and vibrio xuii sp. nov.,
isolated from the marine aquaculture environment (bivalves, fish, rotifers
and shrimps)
F.L. Thompson, Y. Li, B.
Gomez-Gil, C.C. Thompson, B. Hoste, K. Vandemeulebroecke, G.S. Rupp, A.
Pereira, M.M. De Bem, P. Sorgeloos, J. Swings-2003
International Journal of
Systematic and Evolutionary
Microbiology, 53: 245-252
Abstract:
The fluorescent amplified
fragment length polymorphism (FAFLP) groups A5 (21 isolates), A8 (6
isolates) and A23 (3 isolates) distinguished in an earlier paper (Thompson
et al., Syst. Appl; Microbiol., 24: 520-538, 2001) were examined in more
depth. These three groups were phylogenetically related to Vibrio tubiashii,
but DNA-DNA hybridization experiments proved that the three AFLP groups are
in fact novel species. Chemataxonomic and phenotypic analyses further
revealed several differences among the 30 isolates and known Vibrio species.
It is proposed to accommodate these isolates in three novel species, namely
Vibrio neptunius (type strain LMG 20536T; EMBL accession
no.AJ316171: G+C content of the type strain 46.0mol%), Vibrio brasiliensis
(type strain LMG 20546T; EMBL accession no. AJ316172; G+C content
of the type strain 45.9 mol%) and Vibrio xuii (typestrain LMG 21346t;
EMBL accession no. AJ316181; G+C content of the type strain 46.6 mol%).
These species can be differentiated on the basis of phenotypic features,
including fatty acid composition (particularly 14:0 iso, 14:0 iso 3-OH, 16:0
iso, 16:0, 17:0 and 17:1 ω8c), enzyme activities and utilization and
fermentation of various carbon sources.
(Laboratory of Microbiology,
Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium, e-mail: Fabiano.Thompson@Ugent.be)