ENTEROVIBRIO
NORVEGICUS GEN. NOV., SP. Nov., isolated from the gut of turbot
(Scophthalmus maximus) larvae: a new member of the family Vibrionaceae
F.L.
Thompson, B. Hoste, C.C. Thompson, J. Goris, B. Gomez-Gil, L. Huys, P. De
Vos, J. Swings-2002
International Journal of
Systematic and Evolutionary Microbiology, 52 : 2015-2022
Abstract:
Twenty-two isolates originating
from the gut of healthy cultured turbot larvae in Norway were investigated
using a polyphasic approach. Amplified fragment length polymorphism
fingerprinting analysis showed that the isolates have typical patterns and
form two main groups. Phylogenetic analysis revealed that the isolates
belong to the γ-Proteobacteria, with Vibrio hollisae as their closest
neighbour. DNA-DNA hybridization, chemotaxonomic and phenotypic analyses
further proved that these isolates represent a tight novel taxon that
differs from currently described species in the family Vibrionaceae. It is
proposed that these novel isolates be accommodated in a new genus,
Enterovibrio gen. nov., with Enterovibrio norvegicus sp. nov. as the type
species. Isolates were motile by a polar flagellum, positive for oxidase,
catalase, arginine dihydrolase and β-galactosidase, but negative for
the Voges-Proskauer reaction. They produced indole, did not reduce nitrate
and were resistant to the vibriostatic agent O/129. The DNA G+C content of
E. norvegicus was 47.1-47.9 mol%. The type strain is E. norvegicus LMG 19839T
(=CAIM 430T).
(Laboratory of Microbiology,
Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium, e-mail: Fabiano.Thompson@Ugent.be)