Effect of ovarian stage on
plasma vitellogenin and calcium in cultured white sturgeon
J. Linares-Casenave, K.J. Kroll, J.P. Van Eenennaam,
S.I. Doroshov-2003
Aquaculture, 221(1-4): 645-656
Abstract:
We developed an enzyme immunoassay for plasma
vitellogenin (VTG) of white sturgeon Acipenser transmontanus,
cultured in California. The assay, based on polyclonal rabbit antibody to
purified VTG, was tested for specificity and applied on sturgeon broodstocks
(age 4–12 years) reared at three locations. Plasma VTG concentrations were
correlated with total plasma calcium and stage of ovarian maturity. Females
in the previtellogenic ovarian stage and at the onset of yolk deposition had
low VTG concentrations, but plasma VTG and calcium increased after onset of
yolk deposition in the oocytes. Total plasma calcium exhibited a linear
relationship with plasma VTG. Concentrations of both metabolites in plasma
discriminate previtellogenic and vitellogenic females, but fail to
discriminate specific stage of ovarian vitellogenesis. The immunoassay for
plasma VTG or flame photometry for total plasma calcium can be utilized to
segregate vitellogenic females in cultured sturgeon broodstocks.
(Department of Animal Science, University of
California, One Shield Avenue, Davis, CA 95616-8521, USA, e-mail of S.I.
Doroshov: sidoroshov@ucdavis.edu)