Proteolytic enzyme treatment: an improved method for elimination of
egg stickiness in tench, Tinca tinca L., in aquaculture
O. Linhart, D. Gela, M. Flajšhans, M. Rodina-2003
Journal of
Applied Ichthyology, 19(3): 134-137
Abstract:
The aim of the study was to improve the
elimination of egg stickiness in tench as an attempt to increase the egg
hatching rate. The usual desticking procedure involving treatment with milk
solution/clay suspension resulted in a hatching rate of 74.1%. The highest
hatching rate of 87.1% was found with a 10.0 ml L
-1
enzyme treatment; hatching rates of 85.2 and 85.1% were recorded at 15.0 and
5.0 ml L
-1,
but the hatching rate decreased to 80.0% at 20.0 ml L
-1.
anova
showed a significant effect of differing concentrations of enzyme and milk
solution/clay suspension control (P < 0.0015, P < 0.0003)
on the hatching rate and duration of egg incubation until hatching,
respectively. Application of proteolytic alcalase enzyme, Merck EC
3.4.21.14, for desticking of tench eggs also decreased the time of egg
handling from almost 1 h, necessary for traditional milk/clay
desticking, to just 2 min.
(University of South Bohemia, Research
Institute of Fish Culture and Hydrobiology, 389 25 Vodňany, Czech
Republic, e-mail: linhart@vurh.jcu.cz)