Disinfection of striped
trumpeter (Latris lineata) eggs with glutaraldehyde
D.T.
Morehead, P.R. Hart-2003
Aquaculture International, 11(3):
255-260
Abstract:
This study assessed the effect of immersing striped
trumpeter eggs in 0 (control), 200, 400, 800, 1600 or 3200 ppm
glutaraldehyde for 10 minutes, two days before hatching. High concentrations
of glutaraldehyde (1600 and 3200 ppm) resulted in no eggs hatching and only
1% of eggs hatched after treatment with 800 ppm glutaraldehyde. Hatching
success of eggs treated with 0, 200 or 400 ppm glutaraldehyde did not differ
(77 ± 6%, n = 3). However, only 2% of larvae
from the control treatment survived to day 5 post-hatching, compared to 45
and 69%, respectively of the larvae from the 200 and 400 ppm glutaraldehyde
treatment. By day 9 post-hatching, larvae from the 400 ppm glutaraldehyde
treatment had significantly higher survival (59%) than larvae from the 200
ppm glutaraldehyde treatment (28%). Thiosulphate citrate bile salt sucrose
agar (TCBS) medium confirmed the presence of bacteria within the seawater
medium, on all control eggs and on 83% of eggs disinfected with 200 ppm
glutaraldehyde, but no bacterial colonies formed on eggs treated with 400,
800, 1600 or 3200 ppm glutaraldehyde. This study found highest survival of
striped trumpeter larvae from eggs disinfected with 400 ppm glutaraldehyde
and suggests that increased survival was a result of reduced bacterial
loading.
(Marine Research
Laboratories and CRC for Aquaculture, Tasmanian Aquaculture and Fisheries
Institute, University of Tasmania, Hobart, 7001, Australia, e-mail: David.Morehead@utas.edu.au)