Larvae and early post-larvae of Penaeus monodon (Fabricius) experimentally infected with white spot syndrome virus (WSSV) show no significant mortality


K. Yoganandhan, R.B. Narayanan,  A.S. Sahul Hameed-2003

Journal of Fish Diseases, 26(7): 385
Abstract:

The pathogenicity of white spot syndrome virus (WSSV) was tested with different developmental stages of Penaeus monodon, i.e. nauplius, protozoeae, mysis, early post-larvae (PL1-10), late post-larvae (PL11-20) and juveniles. WSSV challenge was done by immersion and oral routes. No disease occurred in the larvae and early post-larvae but they were positive for WSSV by nested polymerase chain reaction (PCR) assay. Significant mortality was observed in late post-larvae and juveniles and both single and nested PCR assays gave positive results with these samples. The results demonstrated that WSSV virulence in P. monodon increases with advancing stages of development and that WSSV infection does not result in disease for larvae and post-larvae younger than PL10.

(Centre for Biotechnology, Anna University, Chennai 600 025, Tamil Nadu, India, e-mail of A.S. Sahul Hameed: cah_sahul@hotmail.com)


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