A rapid non-enzymatic method of DNA extraction for PCR detection of
white spot syndrome virus in shrimp
K.
Yoganandhan, S. Sathish, R.B. Narayanan, A.S. Sahul Hameed-2003
Aquaculture
Research, 34(12):
1093
Abstract:
The
present study describes a simple method of extraction of white spot syndrome
viral DNA (WSSV) from infected shrimp for the polymerase chain reaction
(PCR) detection of WSSV. The DNA preparation using this method was found to
be free from the host DNA, RNA and protein, and is suitable for different
PCR protocols such as single-step PCR, nested PCR and single-tube
semi-nested PCR. This method of extraction has worked successfully for
extracting the WSSV-DNA from different organs (haemolymph, eyestalk,
carapace, head muscle, heart, gills, appendages, heptopancreas, stomach,
intestine, abdominal muscle and tail muscle) of WSSV-infected adult shrimp,
and WSSV-infected larvae and postlarvae.
(Department of Zoology, C. Abdul Hakeem
College, Melvisharam 632 509, Vellore District, Tamil Nadu, India. E-mail of
A.S. Sahul Hameed: cah_sahul@hotmail.com)