The effects of osmolality, cryoprotectant and
equilibration time on striped bass Morone saxatilis sperm motility
S.Y. He, L.C. Woods-2003
Journal of the World Aquaculture Society, 34(3):
255-265 (ISI Current Contents)
Abstract:
Four experiments were designed to evaluate the effects of osmolality,
cryoprotectant, and equilibration time on striped bass sperm motility. In
the first experiment, solutions of NaCl or KCl with osmolalities ranging
from 0 to 700 mmol/kg were tested on sperm activation. Over 60% of the sperm
were activated by isotonic NaCl and KCl solutions with a treatment
osmolality of 350 mmol/kg. Sperm remained motile until osmolality increased
to 600 mmol/kg. In the second and third experiments, Extenders 1, 2 and 3
with osmolalities of 350, 500, and 600 mmol/kg, respectively, were tested.
Sperm samples stored in Extender 2 showed significantly higher (P < 0.01)
sperm motility after 10 min of exposure as well as greater (P < 0.01)
post-thaw motility when compared to samples stored in Extenders I and 3. In
the fourth experiment, two trials were carried out to evaluate the effects
of cryoprotectant and equilibration time. In the first trial, methanol with
a concentration of 5% and 10% yielded the highest (P < 0.05) sperm
motility prior to freezing at all equilibration times examined. However, 5%
DMSO yielded the highest (P < 0.01) post-thaw motility (38 +/- 3.6%).
DMSO with concentrations of 10% and 15% resulted in 17 +/- 2.3% and 6 +/-
1.0% post-thaw motility, respectively. Both methanol and DMA, at all
concentrations tested, resulted in less than 10% post-thaw motility. In the
second trial, four DMSO concentrations with three different equilibration
times were examined. We observed a significant (P < 0.001) interaction
effect between DMSO concentration and equilibration time. Post-thaw motility
was significantly greater (P < 0.01) with a concentration of 5% DMSO at
all equilibration times examined, compared to 1.25, 2.5, and 10% DMSO. An
average post-thaw motility of 40 +/- 2.9% was achieved after 10 min
equilibration using 5% DMSO.
(Univ Maryland, Dept Anim & Avian Sci, College
Pk, MD 20742 USA)