Broodstock rearing and
controlled reproduction of reeves shad Tenualosa reevesii
H.P. Wang, B.X. Xiong, K.J. Wei, H. Yao-2003
Journal
of the World Aquaculture Society, 34(3): 308-318
(ISI Current Contents)
Abstract:
Reeves shad Tenualosa reevesii is one of China's best known anadromous fish,
but is presently on the verge of extinction. There is an urgent need for
conservation of this species through artificial reproduction and culture. In
this study, shad broodstock were held in captivity then were induced to
spawn with serial injections of luteinizing hormone-releasing hormone
analogue (LHRHa) following GtH priming by implantations of
17-methytestosterone (T) combined with environmental manipulations. Induced
maturation of 4, 5, and 6-year-old females reached 83.3%, 87.5%, and 100%,
respectively, while fish in control treatments remained immature. During the
gonadal development priming period, mean serum 17-estradiol levels in the T
treatment were significantly higher than the LHRHa treatment and control
fish. Gonadal development of females was suppressed when their mesenteric
fat index was below 1.5% by April. The effective accumulating temperatures
for female and male maturation were 3,995 +/- 31.4 C/d and 2,278 +/- 8.9
C/d, respectively. Two injections of LHRHa in combination with domperidone
(DOM) and environmental regulations were effective in inducing ovulation.
About 800,000 fertilized ova were obtained (fertilization 75%) in 1995, and
5,000 fry were hatched in 1996. These results are an important step to
develop shad culture and save this endangered species.
(Osu S. Ctr., 1864 Shyville Rd., Piketon OH 45661,
USA)